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Construction of a matrix assisted laser desorption/ionization time-of-flight mass spectrometer

Authors:

RV Coorey ,

Department of Physics, University of Colombo, Colombo 03, Sri Lanka, LK
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TR Ariyaratne,

Department of Physics, University of Colombo, Colombo 03, Sri Lanka, LK
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DDN Barlo Daya,

Department of Physics, University of Colombo, Colombo 03, Sri Lanka, LK
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WMKP Wijayaratna

Department of Physics, University of Colombo, Colombo 03, Sri Lanka, LK
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Abstract

A matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer has been constructed at the Department of Physics, University of Colombo, Sri Lanka. In this mass spectrometer, a solid sample which contains analyte molecules embedded in a suitable matrix compound in excess is exposed to a UV pulsed nitrogen laser source (λ = 337 nm). The desorbed secondary ions from the sample are mass analyzed using the time-of-flight technique. The first MALDI-TOF mass spectra of two peptides namely [tyr8]-bradykinin (1076.2 u) and porcine N-acetyl renin substrate tetradecapeptide (1801.1 u) with the matrix 2,5 di-hydro benzoic acid (154.1 u) have been obtained. Each peptide produced a MH+ peak corresponding to its intact molecular ion with m/q values of 1089 ± 6 and 1794 ± 8 respectively.

doi:10.4038/sljp.v1i0.168

Sri Lankan Journal of Physics, Vol.1,(2000) 33-42

How to Cite: Coorey, R. et al., (2000). Construction of a matrix assisted laser desorption/ionization time-of-flight mass spectrometer. Sri Lankan Journal of Physics. 1, pp.33–42. DOI: http://doi.org/10.4038/sljp.v1i0.168
Published on 17 Dec 2000.
Peer Reviewed

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